NanoBrook 173

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NanoBrook 173

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Features at a glance

Rapid and accurate protein and nanoparticle size distributions
Multimodal & unimodal size distribution software
ISO 13321 and ISO 22412 compliant results
Range > 0.3 nm to 10 Âµm
Customizable reports
Ideal for fast, routine sizing applications in research or quality control
High power 35 mW diode laser
Dynamic light scattering at 173° only
Temperature control, -5 °C to 110 °C
Compact bench top unit, USB connection

Brookhaven's NanoBrook 173 instrument is the go-to-solution for characterization of proteins, antibodies, nanoparticles, macromolecular assemblies and other molecules/particles of small sizes. The NanoBrook 173 uses backscattering detection for the highest sensitivity and accuracy optimized for molecules and particles less than a few tens of nanometers.

Reliable, Accurate, and Accurate Analysis
The new NanoBrook 173 protein and particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two.

Nanoparticles
Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents, if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Sample volumes as small as 50 µL are used. Sample is recoverable. Disposable, polystyrene U-shaped cells are used for aqueous and simple alcohol suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 173.

Speed and low concentration/small size capabilities
The NanoBrook 173 utilizes backscattering to size the proteins/particles by accumulating the correlation function. The smoother the function, the better the results are. For small molecules, the scattered intensity is typically low. The measurement takes longer to get enough statistics from the photons counted to aquire a good results. The backscattering angle makes for a larger scattering volume, measuring more proteins/particles for a quick measurement of their sizes. The advantage of the backscattering is clear for sizes below 10 nm.

Data Presentation
The NanoBrook 173 protein and particle size analyzer offers many choices for data analysis. One choice is the lognormal distribution by: intensity, volume, surface and number. Another choice, the full size distribution (MSD) can be displayed by the same four weighting factors. In the example above, the MSD is presented by volume for lysozyme. The size distribution and cumulative distribution are shown in the table on the right. Lysozyme has a very low polydispersity in this case.

Sample Type	Most globular proteins, nanoparticles, and small polymers in any non-absorbing liquid
Size Range	> 0.3 nm to 10 µm diameter, depending on refractive index and concentration
Sample Cells	50 µL disposable, 1 to 3 mL disposable plastic, glass or quartz cells
Concentration Range	0.1 ppm to 50 mg/mL, depending on refractive index and concentration
Signal Processing	Dynamic Light Scattering, DLS
Correlator	Brookhaven's TurboCorr, multitau research grade with 510 hardware channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range
Precision	± 1% typically
Temperature Control	-5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required.
Condensation Control	Purge facility using dry air, nitrogen preferred
Standard Laser	35 mW red diode laser, nominal 640 nm wavelength
Scattering Angle	173°
Data Presentation	Average & width, lognormal fit, and multimodal size distribution standard
Compliance	ISO 13321 and ISO 22412 compliant results
Power Requirements	100/115/220/240 VAC, 50/60 Hz, 150 Watts
Dimensions	23.3 x 42.7 x 48.1 (HWD)
Weight	15 kg
Environmental Characterisitics	Temperature 10 °C to 75 °C Humidity 0% to 95%, non-condensing
CE Certificate	Class I laser product, EN 60825-1:2001 CDRH